SrasV1, Main, Exploration, bibRecord, 003578

The SARS-Coronavirus Membrane protein induces apoptosis through modulating the Akt survival pathway.

Identifieur interne : 003578 ( Main/Exploration ); précédent : 003577; suivant : 003579

The SARS-Coronavirus Membrane protein induces apoptosis through modulating the Akt survival pathway.

Auteurs : Chak-Ming Chan [République populaire de Chine] ; Cheuk-Wing Ma ; Wood-Yee Chan ; Ho Yin Edwin Chan

Source :

Archives of biochemistry and biophysics [ 0003-9861 ] ; 2007.

RBID : pubmed:17306213

Descripteurs français

KwdFr :
- Animal génétiquement modifié, Animaux, Apoptose (physiologie), Drosophila, Humains, Lignée cellulaire, Phosphorylation, Protéine oncogène v-akt (métabolisme), Protéines de la matrice virale (génétique), Protéines de la matrice virale (métabolisme), Rein (anatomopathologie), Survie cellulaire, Transduction du signal (physiologie), Virus du SRAS (métabolisme).
MESH :
- anatomopathologie : Rein.
- génétique : Protéines de la matrice virale.
- métabolisme : Protéine oncogène v-akt, Protéines de la matrice virale, Virus du SRAS.
- physiologie : Apoptose, Transduction du signal.
- Animal génétiquement modifié, Animaux, Drosophila, Humains, Lignée cellulaire, Phosphorylation, Survie cellulaire.

English descriptors

KwdEn :
- Animals, Animals, Genetically Modified, Apoptosis (physiology), Cell Line, Cell Survival, Drosophila, Humans, Kidney (pathology), Oncogene Protein v-akt (metabolism), Phosphorylation, SARS Virus (metabolism), Signal Transduction (physiology), Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism).
MESH :
- chemical , genetics : Viral Matrix Proteins.
- chemical , metabolism : Oncogene Protein v-akt, Viral Matrix Proteins.
- metabolism : SARS Virus.
- pathology : Kidney.
- physiology : Apoptosis, Signal Transduction.
- Animals, Animals, Genetically Modified, Cell Line, Cell Survival, Drosophila, Humans, Phosphorylation.

Abstract

A number of viral gene products are capable of triggering apoptotic cell death through interfering with cellular signaling cascades, including the Akt kinase pathway. In this study, the pro-apoptotic role of the SARS-CoV Membrane (M) structural protein is described. We found that the SARS-CoV M protein induced apoptosis in both HEK293T cells and transgenic Drosophila. We further showed that M protein-induced apoptosis involved mitochondrial release of cytochrome c protein, and could be suppressed by caspase inhibitors. Over-expression of M caused a dominant rough-eye phenotype in adult Drosophila. By performing a forward genetic modifier screen, we identified phosphoinositide-dependent kinase-1 (PDK-1) as a dominant suppressor of M-induced apoptotic cell death. Both PDK-1 and Akt kinases play essential roles in the cell survival signaling pathway. Altogether, our data show that SARS-CoV M protein induces apoptosis through the modulation of the cellular Akt pro-survival pathway and mitochondrial cytochrome c release.

DOI: 10.1016/j.abb.2007.01.012
PubMed: 17306213

Affiliations:

Le document en format XML

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<term>Humans</term>
<term>Kidney (pathology)</term>
<term>Oncogene Protein v-akt (metabolism)</term>
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<term>SARS Virus (metabolism)</term>
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<front><div type="abstract" xml:lang="en">A number of viral gene products are capable of triggering apoptotic cell death through interfering with cellular signaling cascades, including the Akt kinase pathway. In this study, the pro-apoptotic role of the SARS-CoV Membrane (M) structural protein is described. We found that the SARS-CoV M protein induced apoptosis in both HEK293T cells and transgenic Drosophila. We further showed that M protein-induced apoptosis involved mitochondrial release of cytochrome c protein, and could be suppressed by caspase inhibitors. Over-expression of M caused a dominant rough-eye phenotype in adult Drosophila. By performing a forward genetic modifier screen, we identified phosphoinositide-dependent kinase-1 (PDK-1) as a dominant suppressor of M-induced apoptotic cell death. Both PDK-1 and Akt kinases play essential roles in the cell survival signaling pathway. Altogether, our data show that SARS-CoV M protein induces apoptosis through the modulation of the cellular Akt pro-survival pathway and mitochondrial cytochrome c release.</div>
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The SARS-Coronavirus Membrane protein induces apoptosis through modulating the Akt survival pathway.

The SARS-Coronavirus Membrane protein induces apoptosis through modulating the Akt survival pathway.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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Pour mettre un lien sur cette page dans le réseau Wicri

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